In vitro prothrombin synthesis from a purified precursor protein III. Preparation of an acid-soluble substrate for vitamin K-dependent carboxylase by limited proteolysis of bovine descarboxyprothrombin |
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| Authors: | B.A.M. Soute C. Vermeer M. De Metz H.C. Hemker H.R. Lijnen |
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| Affiliation: | 1. Department of Biochemistry, Biomedical Centre, State University of Limburg, P.O. Box 616, 6200 MD Maastricht The Netherlands;2. Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium |
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| Abstract: | Bovine descarboxyprothrombin and descarboxyfragment-1 can be used as substrates for rat and bovine vitamin K-dependent carboxylase. In both enzyme systems, however, these substrates have a high Km (0.3–0.4 mM). A better substrate (Km = 0.001–0.003 mM) was prepared from bovine descarboxyprothrombin by limited proteolysis with subtilisin Carlsberg. This substrate is called Fragment-Su and is composed of the amino acids 13–29 of descarboxyprothrombin. |
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| Keywords: | Prothrombin synthesis Vitamin K Carboxylase Descarboxyprothrombin (Bovine) |
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