Sulfite: Preferential sulfur source and modifier of CO2 fixation in Chlorella vulgaris |
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Authors: | Gian Franco Soldatini Irmgard Ziegler Hubert Ziegler |
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Institution: | (1) Lehrstuhl für Botanik, Technische Universität München, München 2, Federal Republic of Germany;(2) Institut für Toxikologie und Biochemie der Gesellschaft für Strahlenund Umweltforschung mbH, Arcisstraße 21, D-8000 München 2, Federal Republic of Germany;(3) Present address: Istituto di Chimica Agraria, Università Pisa, Italy |
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Abstract: | Sulfite was added at the time of inoculation to a standard and to a sulfate deficient medium of Chlorella vulgaris. It was not only used as a sulfur source, but besides this, at concentrations <1.0 mmol l–1, the growth yield was enhanced up to 30% compared to sulfate saturated conditions. Higher sulfite concentrations increasingly inhibited cell growth. Growth rate determinations indicated that the enhancement, and the inhibition respectively, were confined to the very beginning of culture growth; the time period during which the sulfite was not yet oxidized (5–10 h). In contrast, an increased CO2 fixation rate/unit of protein, occurring up to 5.0 mmol l–1 sulfite and a shift towards the -carboxylation pathway, are persisting at least during the growth period of 4 days. The preferential uptake of sulfite, also indicated by a marked increase in methionine content of algal protein, presumably causes an increase in thylakoidal sulfolipids, and is such modifying the CO2 fixation.Abbreviations PGA
3-phosphoglyceric acid
- APS
adenosine 5-phosphosulfate
- PEP
phosphoenolpyruvate |
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Keywords: | Chlorella CO2 fixation Growth yield Methionine O2 evolution Sulfolipids Sulfite action |
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