Caveolin-3 Is Associated with the T-Tubules of Mature Skeletal Muscle Fibers |
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Authors: | Evelyn Ralston Thorkil Ploug |
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Institution: | a Laboratory of Neurobiology, NINDS, National Institutes of Health, Bethesda, Maryland, 20892-4062;b Copenhagen Muscle Research Centre, Rigshospitalet, Department of Medical Physiology, The Panum Institute, University of Copenhagen, DK-2200, Copenhagen, Denmark |
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Abstract: | Caveolae are abundant in skeletal muscle and their coat contains a specific isoform of caveolin, caveolin-3. It has been suggested that during muscle development, caveolin-3 is associated with the T-tubules, but that in adult muscle it is found on the plasma membrane only. We have studied the distribution of caveolin-3 in single skeletal muscle fibers from adult rat soleus by confocal immunofluorescence and by immunogold electron microscopy. We found that caveolin-3 occurs at the highest density on the plasma membrane but is also present in the core of the fibers, at the I-band/A-band interface where it is associated with the T-tubules. In neither domain of the muscle surface does caveolin-3 colocalize with the glucose transporter GLUT4 and there is no evidence for internalization of the caveolae in muscle. |
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