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PCR Methods for Identification and Specific Detection of Probiotic Lactic Acid Bacteria |
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| Authors: | María Inés Castellanos Abel Chauvet Alain Deschamps Christian Barreau |
| Institution: | Société Bio-Armor, Zone Industrielle de la Gare, 22940 Plaintel, France, FR Laboratoire de Microbiologie Alimentaire et de Biotechnologie, ISTAB, Université Bordeaux I Nouvelle, Av. des Facultés, 33405 Talence Cedex, France, FR Laboratoire de Génétique Moléculaire des Champignons Filamenteux, UPR CNRS 9026, IBGC, 1, rue Camille Saint-Sa?ns, 33077 Bordeaux Cedex, France, FR |
| Abstract: | Probiotics are defined as “microbes improving animal feed.” Three lactic acid bacteria, previously selected as probiotic for pig feeding, were identified by sequencing the variable V1 region of the 16S rDNA after PCR amplification primed in the flanking constant region. A VR region showing strong nucleotide differences between the three probiotic and the reference strains was delimited. Oligonucleotides specific for each strain were designed. A specific assay for probiotic detection was developed, based on a PCR reaction with three primers. Received: 7 December 1995 / Accepted: 30 January 1996 |
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