Effect of particle size of activated charcoal on separation of Triton X-100 from protein, liver cytosol, and lipoxygenase extracts |
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| Authors: | L Marcus D Prusky |
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| Institution: | 1. College of Geoscience and Survey Engineering, China University of Mining and Technology, Beijing 100083, PR China;2. School of Resources and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, Henan, PR China;3. School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK;1. Plant Nutrition Department, Aula Dei Experimental Station (CSIC), P.O. Box 13034, E-50080, Zaragoza, Spain;2. Instituto de Recursos Naturales y Agrobiología de Sevilla (CSIC), Reina Mercedes 10, E-41012 Sevilla, Spain;3. USDA-ARS Children''s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USA |
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| Abstract: | Triton X-100 was almost completely removed from bovine serum albumin solutions, BALB/c mouse liver extracts, and avocado peel lipoxygenase extracts by stirring the samples for 30 min in the presence of 250-350 mesh activated charcoal. The procedure did not remove protein significantly and did not reduce enzyme activity. At higher charcoal particle sizes, the efficiency of Triton adsorption was decreased and protein adsorption was increased. ElevateD temperatures enhanced Triton and protein adsorption. Adsorption on activated charcoal of 250-350 mesh is a simple and rapid procedure for Triton removal at a ratio of 0.23 g Triton X-100 per gram of activated charcoal. |
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