Production of androgenic plants through Pollen embryogenesis in anther cultures ofBrassica carinata A. Braun |
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Authors: | Renu Arora Sant S Bhojwani |
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Institution: | (1) Department of Botany, University of Delhi, Delhi 110007, India |
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Abstract: | Pollen embryogenesis occurred in anther cultures of two genotypes ofBrassica carinata A. Braun. Pretreatment of anthers at 35°C for 3 or 6 days was essential for the induction of androgenesis on growth regulator-free
culture medium. A combination of sucrose and glucose was better than sucrose alone. None of the pollen embryos germinated
normally. Full plants were raised through adventitious bud differentiation from their hypocotyl. |
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