Identification of tryptophan in leachate of oat hulls (Avena sativa) as a mediator of root growth regulation |
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| Authors: | M.-L. Hä ggquist,L. Strid,K.-O. Widell,C. Liljenberg |
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| Affiliation: | Dept of Plant Physiology, Univ. of Göteborg, Carl Skottsbergs gata 22, S-413 19 Göteborg;;Dept of Biochemistry and Biophysics, Chalmers Univ. of Technology and Univ. of Göteborg, Kemigården 3, S-412 96 Göteborg, Sweden. |
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| Abstract: | Leachate of oat hulls ( Avena sativa L. cv. Sang) inhibits root elongation and causes swollen roots and abundant root hair formation. The active substance was isolated by column chromatography and thin layer chromatography (TLC) systems. High performance liquid chromatography (HPLC) revealed 3 peaks, one of which corresponded to the substance responsible. The latter was identified as tryptophan by means of its UV spectrum, amino acid analysis, nuclear magnetic resonance spectrometry (NMR) and mass spectrometry (MS). |
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| Keywords: | Avena sativa high performance liquid chromatography indole-3-acetic acid mass spectrometry nuclear magnetic resonance spectrometry oat root hairs tryptophan |
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