基于UPLC-QTOF-MS技术分析多脂鳞伞特征性成分及体外抗肿瘤作用

多脂鳞伞是一种较为珍贵的药食用真菌。对其化学成分及体外抗肿瘤作用进行了研究。通过UPLC-QTOF-MS法、电喷雾离子源（ESI）及负离子全扫描模式测定了人工栽培多脂鳞伞的化学成分组成;体外培养HepG-2、A549、Hela及MCF-7,用不同质量浓度多脂鳞伞乙醇提物处理细胞,应用CCK-8法及流式细胞术进行细胞毒性测试,并应用流式细胞术选择HepG-2细胞系以进一步评估其对HepG-2细胞凋亡分析。结果表明：经UPLC-QTOF-MS分析,从多脂鳞伞子实体分析得到的化学成分与食药用菌成分数据库中的38种成分相吻合,其中棕榈酸与9E,12E-octadecadienoic acid含量最高,甾醇类化合物与多糖类化合物占比较高;多脂鳞伞乙醇提取物对HepG-2细胞毒性最强,其浓度为50μg/mL时凋亡率能够达到(23.71±1.59)%。多脂鳞伞具有多种功能性化学成分,并具有潜在的抗肿瘤应用价值,初步断定其抗肿瘤活性是功能性成分通过诱导细胞凋亡实现的。

Chemical components of Pholiota adiposa analyzed by UPLC-QTOF-MS and their antitumor effects

The chemical constituents and anti-tumour activity in vitro of cultivated Pholiota adiposa were studied by using UPLC-QTOF-MS method. HepG-2, A549, HeLa and MCF-7 cells were cultured in vitro and treated with different concentrations of ethanol extracts of Pholiota adiposa (EPA), and the cytotoxicity was assayed by CCK-8 method. Flow cytometry was used for HepG-2 cells, apoptosis analysis via annexin VFITC/PI double staining. A total of 38 components is consistant with that recorded in the chemical composition database. The content of palmitic acid and 9E,12E-octadecadienoic acid was the highest, and sterol compounds and polysaccharide compounds accounted for a relatively high proportion. EPA showed the strongest cytotoxicity to HepG-2 cells, and the apoptosis rate was up to (23.71±1.59)% under the concentration of 50μg/mL. Pholiota adiposa has a variety of functional chemical constituents and potential anti-tumor application value. It is preliminally concluded that the anti-tumor activity is realized by the functional constituents through inducing apoptosis.