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野生桑树桑黄和杨树桑黄化学成分及抗氧化活性比较
引用本文:吕国英,宋婷婷,蔡为明,张作法.野生桑树桑黄和杨树桑黄化学成分及抗氧化活性比较[J].菌物学报,2021,40(7):1833-1843.
作者姓名:吕国英  宋婷婷  蔡为明  张作法
作者单位:浙江省农业科学院园艺研究所 浙江 杭州 310021
基金项目:浙江省自然科学基金(LQY20C150001);浙江省农业新品种选育重大科技专项(2016C02057-8)
摘    要:比较了野生桑树桑黄和杨树桑黄子实体乙醇提取物抗氧化活性和化学成分的差异,探讨其高抗氧化能力的来源。以二苯基三硝基苯肼自由基(DPPH)清除率、超氧阴离子清除率和β-胡萝卜素漂白实验作为抗氧化的指标比较其抗氧化活性差异。结果表明,桑树桑黄和杨树桑黄均具有很强的抗氧化活性,杨树桑黄的抗氧化活性显著强于桑树桑黄;杨树桑黄醇提物的总黄酮和总多酚含量均高于桑树桑黄醇提物。通过超高效液相色谱串联三重四级杆飞行时间质谱法(UPLC-Triple-TOF-MS)比较了桑树桑黄和杨树桑黄乙醇提取物成分差异,桑树桑黄中共鉴定出19种多酚类物质,杨树桑黄中除了与桑树桑黄中相同的19种物质,还另外分析出3种多酚类物质。

关 键 词:桑黄  抗氧化  化学成分  超高效液相色谱串联三重四级杆飞行时间质谱法  
收稿时间:2020-12-15

Comparative study of chemical components and antioxidant activities of wild Sanghuangporus sanghuang and Sanghuangporus vaninii
Authors:LV Guo-Ying  SONG Ting-Ting  CAI Wei-Ming  ZHANG Zuo-Fa
Institution:Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China
Abstract:Antioxidant activities and chemical components of ethanol extracts of fruit bodies of wild Sanghuangporus sanghuang and S. vaninii were compared. DPPH scavenging activity, superoxide anion scavenging activity and β-carotene bleaching assay were used to compare the antioxidant activity. The results showed that both S. sanghuang and S. vaninii had strong antioxidant activity, and the antioxidant activity of S. vaninii was significantly stronger than that of S. sanghuang. The content of total flavonoid and total polyphenol in the ethanol extract of S. vaninii were higher than that of S. sanghuang. Ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry (UPLC-Triple-TOF-MS) was used to compare the differences of chemical components in the ethanol extracts. Nineteen phenolic compounds were common to S. sanghuang and S. vaninii, and additional three phenolic compounds were identified from S. vaninii.
Keywords:Sanghuangporus  antioxidant  chemical components  ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry  
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