硒对镉胁迫下大球盖菇菌丝生长的影响

选用大球盖菇Stropharia rugosoannulata为试验材料,分析在镉胁迫处理下（2mg/L）添加不同浓度硒（0、6、15、20mg/L）对大球盖菇菌丝生长的影响,初步研究了施加硒对镉胁迫下大球盖菇菌丝生长抑制的缓解效应和作用机制。结果表明：镉对大球盖菇生长速度和生物量具有明显抑制作用,镉造成了大球盖菇渗透调节物质、过氧化氢和丙二醛含量显著增加;添加硒后,镉对大球盖菇菌丝的生长速度、生物量抑制作用均有所改善,且增加了渗透调节物质,促进镉胁迫下大球盖菇渗透调节能力;硒诱导抗氧化酶的活性上调,提高活性氧清除能力,降低了菌丝中丙二醛和过氧化氢的含量,缓解细胞膜过氧化程度,增强了大球盖菇对镉的耐受性。本研究重点探讨外源添加硒对镉胁迫下大球盖菇菌丝生长、生理特性及抗氧化胁迫能力的影响,为进一步研究硒在食用菌抗镉栽培中的应用奠定了一定的理论基础。

Effects of exogenous selenium on the mycelial growth of Stropharia rugosoannulata under cadmium stress

Stropharia rugosoannulata was selected as the test material, and the effects of different concentrations of selenium (0, 6, 15, 20mg/L) on the growth characteristics of mycelia under cadmium stress (2mg/L) were analyzed. Results showed that compared with the untreated control, the growth rate and biomass of S. rugosoannulata under cadmium treatment were inhibited, but the osmotic adjustment substances, hydrogen peroxide and malondialdehyde content increased significantly. Under combined treatment of selenium and cadmium, the growth rate and biomass of S. rugosoannulata mycelia were improved, and osmotic adjustment substances were significantly increased, promoting the osmotic adjustment ability of S. rugosoannulata under cadmium stress. Selenium induced up-regulation of antioxidant enzyme activities, improved the ability of scavenging active oxygen, reduced the content of malondialdehyde and hydrogen peroxide in the mycelia, relieved the degree of peroxidation of the cell membrane, and enhanced the tolerance of S. rugosoannulata to cadmium stress. This research focuses on the effects of selenium on the growth, physiological characteristics and anti-oxidative stress of S. rugosoannulata under cadmium stress, and theoretically lays a basis for further applying selenium in cadmium-resistant cultivation of edible fungi.