羧甲基茯苓多糖的水媒法制备及其免疫活性研究

羧甲基茯苓多糖carboxymethyl-pachymaran (CMP)具有良好的免疫调节活性,传统方法使用两步工序在醇相体系中制得,有机溶剂消耗大、成本高.本研究报道一种新制备方法,将提取茯苓多糖以及茯苓多糖与氯乙酸的醚化反应整合在一个碱液体系中完成.动物实验表明,新方法制备的CMP能提高环磷酰胺诱导的免疫低下小鼠...

Carboxymethyl-pachymaran from the fruiting body of Poria cocos:preparation in aqueous system and its immunomodulation activity

Traditionally, the immunomodulatory carboxymethyl-pachymaran (CMP) polysaccharide was prepared in a two-step process in alcohol solution. However, this process consumes a large amount of organic solvents and is not cost-effective. In the present study, a new method for preparing CMP is reported. The extractions of polysaccharides from the fruiting body ofPoria cocos and the etherification of polysaccharides with chloroacetic acid were integrated into one-step process in aqueous alkaline solution. All the same, the CMP prepared by the new method improved cellular and humoral immune responses in cyclophosphamide-induced immunosuppressed mice and exhibited anti-tumor activity in S180-bearing mice. Oral acute toxicological results demonstrate that CMP is a non-toxic substance, and suitable for the development of functional foods in food industry.