华重楼灰霉病菌灰葡萄孢ITS分型及SSR遗传多样性分析

华重楼灰霉病在湖北和湖南两省多有发生,己对华重楼药材生产构成了严重威胁.为明确该病菌的遗传多样性水平,本研究对分离自两省10个地区的92株华重楼灰霉病菌进行了 rDNA-ITS分子鉴定,同时采用SSR-PCR技术进行了遗传多样性分析.rDNA-ITS分子鉴定结果表明92株病原菌均为灰葡萄孢Botrytis cinere...

ITS and SSR-PCR analyses reveal the genetic diversity of Botrytis cinerea strains collected from Paris polyphylla var. chinensis

Botrytis disease is the most common and serious disease on Paris polyphylla var.chinensis in Hubei and Hunan provinces.In order to clarify the genetic diversity level of the pathogen Botrytis cinerea,92 isolates from 10 regions of Hubei and Hunan provinces were analyzed by rDNA-ITS molecular identification and simple sequence repeats(SSR-PCR)molecular marker technique.rDNA-ITS molecular identification showed that these isolates were all B.cinerea and ITS phylogeny tree indicated significant genetic diversity.SSR-PCR diversity analysis showed that 109 alleles were detected by 9 primer pairs,and observed number of alleles and effective number of alleles average 12 and 3.6589 respectively.Shannon’s diversity index was1.5048,and polymorphism information content 0.6196.For the investigated 10 regions,the values of effective number of alleles ranged from 1.622 to 3.971,with Shannon’s diversity index of 0.527-1.582,observed heterozygosity of 0.148-0.593,and expected heterozygosity of0.321-0.708.The UPGMA phylogenetic tree based on Nei’s genetic distance showed that fungal isolates from 10 regions were divided into four groups.Gene flow value between populations was 0.19-4.67,with genetic differentiation value of 0.047-0.638,genetic identity value of0.1531-0.9679,and genetic distance value of 0.0327-1.8766.In summary,the populations of B.cinerea on Paris polyphylla var.chinensis in Hubei and Hunan provinces were genetically diverse,and the results may benefit the control of the disease in these provinces.