虫草素联合阿霉素干预乳腺癌细胞增殖及转移作用

研究了虫草素联合阿霉素在体外抑制三阴性乳腺癌MDA-MB-231细胞增殖及转移作用,评估了联合用药的作用效应,为虫草素在临床应用上增强抗乳腺癌作用提供了科学数据。研究结果表明,联合用药比单独用药作用效果更明显,根据Chou-Talalay法显示出在80μmol/L虫草素联合1μmol/L阿霉素的条件下,联合用药协同作用最优,CI值为0.665,细胞抑制率达到60.31%±1.06%;与对照组相比,平板克隆形成实验证明联合用药显著抑制细胞增殖,克隆形成率仅为7.03%±1.19%;显微观察细胞形态变化表明联合用药明显影响细胞生长;Hochest 33258染色、DNA Ladder发现联合用药对细胞凋亡诱导作用更显著,细胞凋亡率可达78.52%±11.18%;细胞划痕愈合实验检测联合用药显著抑制细胞迁移,细胞迁移率仅为18.82%±2.43%。本研究确证虫草素可协助阿霉素治疗乳腺癌的增敏作用。

Effects of cordycepin combined with doxorubicin on proliferation and metastasis of breast cancer cells

The inhibitory effect of cordycepin combined with doxorubicin on proliferation and metastasis of triple negative breast cancer MDA-MB-231 cells in vitro was studied, and the effect of compound remedy was evaluated, for the purposes of enhancement of anti-breast cancer effect of cordycepin in clinical application. The results showed that cordycepin and doxorubicin compound was more obviously effective than single drug treatment. According to Chou-Talalay method, 80μmol/L cordycepin combined with 1μmol/L doxorubicin manifests the best synergistic effect, and the CI value is 0.665, and the cell inhibition rate reaches 60.31%±1.06%. Compared with the experimental control, such a drug combination significantly inhibited cell proliferation, and the plate clone formation rate was only 7.03%±1.19%. Microscopic observation showed that the compound remedy significantly affected cell growth. Hochest 33258 staining and DNA Ladder indicate that the compound remedy has a more significant effect on the induction of apoptosis, and the apoptosis rate can reach 78.52%±11.18%. The cell scratch healing test detected that the compound significantly inhibited cell migration, and the cell migration rate was only 18.82%±2.43%. This study confirms that cordycepin can assist doxorubicin in the sensitization of breast cancer.