预热处理金针菇蛋白对猪肉肌原纤维蛋白凝胶特性的影响

本文研究预热处理(preheat treatment,PT)金针菇蛋白(Flammulina filiformis protein,FFP)对肌原纤维蛋白(myofibrillar protein,MP)凝胶特性的影响.试验将FFP在65、75、85和95℃下分别预热处理30、60、90和120min,以蛋白溶解度、总...

Effects of preheat treatment Flammulina filiformis protein on the properties of pork myofibrillar protein gel

The effect of preheat treatment (PT) Flammulina filiformis protein (FFP) on the gel properties of myofibrillar protein (MP) was investigated. FFP was preheated at a series of temperature (65, 75, 85 and 95°C) for different time (30, 60, 90 and 120min), respectively. The optimal temperature and time were determined based on protein solubility, total sulfhydryl content and surface hydrophobicity. Preheat treatment FFP (PT-FFP) was mixed with MP at different ratios (0:10, 1:9, 2:8, 3:7, 4:6, M/M) to prepare mixed gels with total protein concentration of 40mg/mL. The effects of PT-FFP on MP gel properties were investigated on the basis of gel intensity, water holding capacity, rheological properties and microstructure. The results showed that solubility and surface hydrophobicity of FFP were the highest and the total sulfhydryl content was the lowest when FFP was preheated at the optimum of 75°C for 60min. PT-FFP prepared under the optimum condition was then mixed with MP at different ratios to prepare gels. It was found that PT-FFP well improved the gel intensity and water holding capacity of the mixed gels as compared with untreated FFP (P<0.05). Notably, the gel intensity and water holding capacity of the mixed gels reached the highest values of 121.38g and 85.25% respectively at the PT-FFP and MP mixed ratio of 1:9. The rheological results also showed that PT-FFP increased the elastic modulus Gʹ and loss modulus G″ of the mixed gel. Scanning electron microscope observation showed that the gel of PT-FFP mixed with MP had stronger continuity and more dense structure. In summary, FFP pre-treated at 75°C for 60min mixed with MP could improve the gel properties and rheological properties of myofibrillar protein at the mixed ratio of 1:9.