| 蛋白激酶PRKX对人肝癌细胞粘附和迁移能力的影响 |
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| 引用本文： | 胡启凯,任斌. 蛋白激酶PRKX对人肝癌细胞粘附和迁移能力的影响[J]. 中国组织化学与细胞化学杂志, 2013, 22(3): 230-233 |
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| 作者姓名： | 胡启凯 任斌 |
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| 作者单位： | 胡启凯 (山西中医学院体育部,太原,030024)； 任斌 (山西中医学院体育部,太原,030024)； |
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| 摘 要： | 目的观察蛋白激酶PRKX对人肝癌细胞SMMC-7721粘附和迁移能力的影响。方法采用脂质体转染的方法,将PRKX表达质粒转染到SMMC-7721细胞中,蛋白印迹方法鉴定转染前后PRKX蛋白的表达。细胞-基质粘附实验测定对照组和PRKX转染组SMMC-7721细胞的粘附能力。细胞迁移实验测定对照组和PRKX转染组SMMC-7721细胞的迁移能力。结果 SMMC-7721细胞转染组PRKX蛋白的表达增加,SMMC-7721细胞转染组的粘附能力和迁移能力均较对照组增加。结论 PRKX可增加人肝癌细胞SMMC-7721的粘附和迁移能力。
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| 关 键 词： | PRKX 肝癌细胞株 细胞粘附 细胞迁移 |
Effects of PRKX on adhesion and migration of human hepatocellular carcinoma cells |
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| Hu Qikai,Ren Bin. Effects of PRKX on adhesion and migration of human hepatocellular carcinoma cells[J]. Chinese Journal of Histochemistry and Cytochemistry, 2013, 22(3): 230-233 |
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| Authors: | Hu Qikai Ren Bin |
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| Affiliation: | (Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China) |
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| Abstract: | Objective To investigate the possible effect of PRKX on the adhesion and migration of the hu- man hepatocellular carcinoma cell line SMMC-7721. Methods The SMMC-7721 cells were transfected with pDONR223-PRKX with Lipofeetamine 2000. The expression of PRKX protein in SMMC-7721 cells was i- dentified by Western blot. The adhesion of SMMC-7721 cells was studied by cell-matrix adhesion assay. Cell migration assay was used to determine the changes in migration of SMMC-7721 cells. Results The ex pression of PRKX protein in SMMC-7721 cells was increased after pDONR223-PRKX transfection. The adhesion and migration of SMMC-7721 cells were enhanced in the transfected group compared with those of control group. Conclusion PRKX increases the adhesion and migration ability of human hepatocellular carcinoma cell line SMMC-7721. |
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| Keywords: | PRKX Hepatocellular carcinoma cell line Cell adhesion Cell migration |
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