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Eleven novel microsatellite markers developed from the living fossil Ginkgo biloba (Ginkgoaceae)
Authors:Xiao-Ling Yan  Ye-Ye Chen  Bi-Cai Guan  Cheng-Xin Fu
Institution:(1) Laboratory of Systematic and Evolutionary Botany, College of Life Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China;
Abstract:Ginkgo biloba L. (Ginkgoaceae), a famous and widely cultivated plant, is often referred to as a living fossil. Here we isolated and characterized 11 polymorphic microsatellite loci from G. biloba using a modified biotin-capture method. These loci showed high levels of allelic diversity, alleles sizes ranged from 4 to 16 per locus with polymorphism information content ranging from 0.432 to 0.909 (average = 0.752). The observed and expected heterozygosities ranged from 0.208 to 0.708 (average = 0.484) and from 0.501 to 0.934 (average = 0.795), respectively. These microsatellite markers can be used as genetic markers for further studies on genetic diversity, genetic structure and the gene flow among populations of this species.
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