Approaches for refining heterologous protein production in filamentous fungi |
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Authors: | Ruchika Sharma Meenu Katoch P S Srivastava G N Qazi |
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Institution: | (1) Biotechnology Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, 180001, India;(2) Department of Biotechnology, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India; |
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Abstract: | Fungi combine the advantages of a microbial system such as a simple fermentability with the capability of secreting proteins
that are modified according to a general eukaryotic scheme. Filamentous fungi such as Aspergillus niger efficiently secrete genuine proteins but the secretion of recombinant proteins turned out be a difficult task. Aspergillus niger is an attractive organism because of its high secretion capacity and is frequently used as a model organism. Whereas high
production yields can be obtained when homologous proteins are expressed, much lower amounts are obtained with the production
of heterologous proteins. To fully exploit the potential of filamentous fungi, understanding of the molecular genetics, their
physiology, and the glycosylation metabolism has to be investigated and clarified in more detail. This review summarizes recent
developments in heterologous protein production by filamentous fungi and also generalizes the possibilities of improving the
protein production by various genetic and bioprocessing approaches, thereby easing recognition of filamentous fungi as a relevant
and reliable expression platform. |
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