Tissue-specific secretory proteins of the salivary glands of Chironomus thummi An electrophoretic and immunochemical analysis |
| |
Authors: | E P Kopantzev E I Karakin I I Kiknadze |
| |
Institution: | (1) Siberian Department of the USSR Academy of Sciences, Institute of Cytology and Genetics, 630090 Novosibirsk, USSR |
| |
Abstract: | The salivary proteins of Chironomus thummi larvae were separated by SDS gel electrophoresis and characterized by immunological techniques. As a result, five protein fractions (sp-220, sp-180, sp-35, sp-18, and sp-16) with molecular weights Mr=220000, 180000, 35000, 18000 and 16000 were identified in 6%–20% polyacrylamide gradient gels. In addition, three giant proteins fractions (molecular weights exceeding Mr=800000) were detected in composite polyacrylamide-agarose gels. Crossed immunoelectrophoresis allowed us to identify five immunochemically dissimilar organ-specific antigen fractions in the salivary gland secretion. Data were obtained indicating that the protein fractions, sp-220, sp-180, sp-35, sp-18, and sp-16, are immunochemically and structurally similar. The giant secretory proteins and the secretory fractions with low molecular weights were found to be immunochemically unrelated. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|