Monoclonal antibody with specificity to mitotic chromosomes of primates |
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| Authors: | Frances M. Davis Rolf-D. Wegner Potu N. Rao |
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| Affiliation: | 1. Basic Department, Air Force Engineering University, Xi’an, Shanxi 710051, PR China;2. State Key Laboratory of Solidification Processing, Center of Advanced Lubrication and Seal Materials, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, PR China;1. Institute of Neuroradiology, University Hospital Zurich, Zurich, Switzerland;2. Department of Psychiatry and Psychotherapy, University Hospital Zurich, Zurich, Switzerland;3. Center of MR-Research, University Children׳s Hospital Zurich, Zurich, Switzerland;4. Neural Systems Group, Massachusetts General Hospital, Boston, MA, USA;5. Psychiatric University Hospital, University of Bern, Bern, Switzerland;6. Department of Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA;7. Center of Education and Research (COEUR), Psychiatric Services of the County of St. Gallen-North, Wil, Switzerland |
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| Abstract: | Fusion of a cell in mitosis with a cell in interphase results in the condensation of chromatin in the interphase nucleus into chromosomes. Premature chromosome condensation is caused by certain proteins, called mitotic factors, that are present in the mitotic cell and are localized on chromosomes. Extracts from mitotic cells were used to immunize mice to produce monoclonal antibodies specific for cells in mitosis. Among the antibodies obtained, the MPM-4 antibody defines a 125-kD polypeptide antigen located on mitotic chromosomes by indirect immunofluorescence. Although the polypeptide antigen is present in approximately equal concentrations in extracts of interphase cells and mitotic cells, as revealed by immunoblots, it cannot be detected cytologically in the former. Cell fractionation experiments showed that the 125-kD antigen is found in the cytoplasm of interphase cells and metaphase cells, but is concentrated in fractions containing metaphase chromosomes, although not detectable in interphase nuclei. Even though the antigen is apparently primate-specific, it binds to mitotic chromosomes and prematurely condensed chromosomes in human-rodent cell hybrids without regard to the species of origin of the mitotic inducer. The presence of the antigen in the cytoplasm of interphase cells and the chromosomes of mitotic cells suggests a relationship between the presence of the antigen on chromosomes and the process of chromosome condensation and decondensation. |
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