A simple method for the isolation of adrenal chromaffin granules on a large scale |
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| Authors: | A. D. Smith and H. Winkler |
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| Affiliation: | Department of Pharmacology, University of Oxford |
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| Abstract: | Highly purified chromaffin granules can be obtained from homogenates of either ox, pig, horse or rat adrenal medullae by ultracentrifugation of the large-granule fraction layered on 1.6m-sucrose solution, by using angle-head rotors. The chromaffin granules are obtained as a pink sediment that is only slightly contaminated by mitochondria and lysosomes. |
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