The value of mutants unable to carry out photorespiration |
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Authors: | Ray D. Blackwell Alan J. S. Murray Peter J. Lea Alan C. Kendall Nigel P. Hall Janice C. Turner Roger M. Wallsgrove |
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Affiliation: | (1) Department of Biological Sciences, University of Lancaster, LA1 4YQ Lancaster, UK;(2) Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts, UK |
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Abstract: | Manipulation of the CO2 concentration of the atmosphere allows the selection of photorespiratory mutants from populations of seeds treated with powerful mutagens such as sodium azide. So far, barley lines deficient in activity of phosphoglycolate phosphatase, catalase, the glycine to serine conversion, glutamine synthetase, glutamate synthase, 2-oxoglutarate uptake and serine: glyoxylate aminotransferase have been isolated. In addition one line of pea lacking glutamate synthase activity and one barley line containing reduced levels of Rubisco are available. The characteristics of these mutations are described and compared with similar mutants isolated from populations of Arabidopsis. As yet, no mutant lacking glutamine synthetase activity has been isolated from Arabidopsis and possible reasons for this difference between barley and Arabidopsis are discussed. The value of these mutant plants in the elucidation of the mechanism of photorespiration and its relationships with CO2 fixation and amino acid metabolism are highlighted.Abbreviations GS cytoplasmic glutamine synthetase - GS2 chloroplastic glutamine synthetase - PFR Photon fluence rate - Rubisco Ribulose-1,5-bisphosphate carboxylase/oxygenase - RuBP Ribulose-1,5-bisphosphate - SGAT serine:glyoxylate aminotransferase |
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Keywords: | Hordeum mutants nitrogen metabolism photosynthesis photorespiration Pisum |
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