Typical methanogenic inhibitors can considerably alter bacterial populations and affect the interaction between fatty acid degraders and homoacetogens |
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Authors: | Kewei Xu He Liu Xiufen Li Jian Chen Aijie Wang |
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Institution: | (1) Laboratory of Environmental Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu Province, People’s Republic of China;(2) School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, 214122, People’s Republic of China;(3) State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China; |
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Abstract: | The effects of two typical methanogenic inhibitors 2-bromoethanesulfonate (BES) and chloroform (CHCl3)] on the bacterial populations were investigated using molecular ecological techniques. Terminal restriction fragment length
polymorphism analyses (T-RFLP) in combination with clone library showed that both the toxicants not only inhibited methanogenic
activity but also considerably altered the bacterial community structure. Species of low % G + C Gram-positive bacteria (Clostridiales), high % G + C Actinomycetes, and uncultured Chloroflexi showed relatively greater tolerance of CHCl3, whereas the BES T-RFLP patterns were characterized by prevalence of Geobacter hydrogenophilus and homoacetogenic Moorella sp. In addition, due to indirect thermodynamic inhibition caused by high hydrogen partial pressures, the growth of obligately
syntrophic acetogenic Syntrophomonas and Syntrophobacter was also affected by selective inhibition of methanogenesis. Interestingly, by comparing the fermentative intermediates detected
in BES- and CHCl3-treated experiments, it was furthermore found that when methanogenesis is specifically inhibited, the syntrophic interaction
between hydrogen-producing fatty acid degraders and hydrogen-utilizating homoacetogens seemed to be strengthened. |
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