Characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines |
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Authors: | Aruliah Rajasekar Balakrishnan Anandkumar Sundaram Maruthamuthu Yen-Peng Ting Pattanathu K S M Rahman |
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Institution: | (1) Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117576, Singapore;(2) Department of Biotechnology, Sourashtra College, Madurai, 625004, India;(3) Biocorrosion Group, Corrosion Protection Division, Central Electrochemical Research Institute, Karaikudi, 630 006, India;(4) Chemical and Bioprocess Engineering Group, School of Science and Technology, University of Teesside, Middlesbrough, TS13BA, Tees Valley, UK; |
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Abstract: | Microbiologically influenced corrosion is a problem commonly encountered in facilities in the oil and gas industries. The
present study describes bacterial enumeration and identification in diesel and naphtha pipelines located in the northwest
and southwest region in India, using traditional cultivation technique and 16S rDNA gene sequencing. Phylogenetic analysis
of 16S rRNA sequences of the isolates was carried out, and the samples obtained from the diesel and naphtha-transporting pipelines
showed the occurrence of 11 bacterial species namely Serratia marcescens ACE2, Bacillus subtilis AR12, Bacillus cereus ACE4, Pseudomonas aeruginosa AI1, Klebsiella oxytoca ACP, Pseudomonas stutzeri AP2, Bacillus litoralis AN1, Bacillus sp., Bacillus pumilus AR2, Bacillus carboniphilus AR3, and Bacillus megaterium AR4. Sulfate-reducing bacteria were not detected in samples from both pipelines. The dominant bacterial species identified
in the petroleum pipeline samples were B. cereus and S. marcescens in the diesel and naphtha pipelines, respectively. Therefore, several types of bacteria may be involved in biocorrosion arising
from natural biofilms that develop in industrial facilities. In addition, localized (pitting) corrosion of the pipeline steel
in the presence of the consortia was observed by scanning electron microscopy analysis. The potential role of each species
in biofilm formation and steel corrosion is discussed. |
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