| 野油菜黄单胞菌S-152遗传转移系统的建立及外源性内切葡聚糖酶基因在其体内的表达 |
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| 引用本文： | 蔡,勉,刘纯强,高培基CAI Mian LIU Chuan-Qiang GAO Pei-Ji.野油菜黄单胞菌S-152遗传转移系统的建立及外源性内切葡聚糖酶基因在其体内的表达[J].遗传,1994,16(1):40-44. |
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| 作者姓名： | 蔡 勉 刘纯强 高培基CAI Mian LIU Chuan-Qiang GAO Pei-Ji |
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| 作者单位： | 山东大学微生物研究所 济南 250100 Institute of Microbiology, Shandong University, jinan 250100) |
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| 摘 要： | (通过细胞接合的方式将广宿主质粒pRK404、pKT230和RP4分别转入野油菜黄单胞菌S-152中，其转移频率均在10-4数量级上，从而建立起了S-152的转移系统。用同法将由载体质粒pRK404和白纹黄单胞菌XA1-1的CMC酶基因构成的重组质粒pND82转入到S-152中并得到了表达，使S-152(pND82)的胞内CMC酶酶活比出发菌株S-152提高了1倍。The wide-host-range plasmid vectors pRK404 and pKT230 were transferred into a recipient strain S-152 respectively by conjugation with the aid of the helper plasmid pRK2013 at frequencies of 10-4, and a genetic transfer system for S-152 was developed. A recombinant plasmid pND82, constructed by combining the plasmid vector pRK404 with a chromosomal DNA fragment coding for CMCase from XA1-1, was introduced into S-152 by this transfer system.As a result, the intracellular CMCase activity of S-152 (pND82) increases 1 time in comparison with S-152.
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| 关 键 词： | 野油菜黄单胞菌 细胞接合 内切葡聚糖酶 基因表达Xanthomonas campestris 遗传转移系统 |
Development of Genetic transfer System and Expression of an Extraneous Endoglucanase gene in xanthomonas s-152 |
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| CAI Mian,LIU Chuan-Qiang,GAO Pei-Ji.Development of Genetic transfer System and Expression of an Extraneous Endoglucanase gene in xanthomonas s-152[J].Hereditas,1994,16(1):40-44. |
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| Authors: | CAI Mian LIU Chuan-Qiang GAO Pei-Ji |
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