Transient change of organization of vimentin filaments during mitosis as demonstrated by a monoclonal antibody |
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Authors: | Werner W. Franke Christine Grund Caecilia Kuhn Veli-Pekka Lehto Ismo Virtanen |
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Affiliation: | 1. Division of Membrane Biology and Biochemistry, Institute of Cell and Tumour Biology, German Cancer Research Centre, D-6900 Heidelberg, Federal Republic of Germany;2. Department of Pathology, University of Helsinki, Helsinki, Finland |
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Abstract: | A monoclonal antibody specific for vimentin is described which, by immunofluorescence and immunoelectron microscopy, decorates fibrillar and/or granular structures in mitotic and early postmitotic cells but does not react with vimentin filaments of interphase stages of various cultured cells (rat vascular smooth muscle-derived cell line RVF-SM; SV40-transformed human fibroblasts; bovine kidney epithelial cells of line MDBK). These observations indicate that the organization of vimentin filaments varies during the cell cycle, undergoing a perimitotic change of filament organization. These changes of vimentin filaments are described in relation to those reported for cytokeratin filaments of various epithelial and carcinoma cells. The possible functional implications of filament protein rearrangements both during the cell cycle and in cell differentiation processes are discussed. |
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Keywords: | To whom offprint requests should be sent. Address: Institute of Cell and Tumor Biology German Cancer Research Center Im Neuenheimer Feld 280 D-6900 Heidelberg Federal Republic of Germany. |
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