Cloning of the gene coding for chitobiase ofSerratia marcescens |
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| Authors: | Hadar Kless Yaron Sitrit Ilan Chet Amos B. Oppenheim |
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| Affiliation: | (1) The Otto Warburg Center for Biotechnology, Faculty of Agriculture, Hebrew University, Rehovot, Israel;(2) Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel |
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| Abstract: | Summary A λ phage DNA library ofSerratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase.Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growingE. coli cells the chitobiase enzyme was found to be secreted into the periplasm. |
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| Keywords: | Serratia marcescens Chitobiase |
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