Detection of protein-dodecyl sulfate complexes with pinacryptol yellow following electrophoretic separation

A rapid technique for the location of proteins separated electrophoretically in the presence of sodium dodecyl sulfate in just the sample solution is described. This procedure involved the use of pinacryptol yellow to locate the protein-dodecyl sulfate complex in the polyacrylamide gel that previously had been subjected to electrophoresis. With pinacryptol yellow as a means of visualization, the time required for the detection of the protein-dodecyl sulfate bands was greatly reduced due to the elimination of the lengthy staining and destaining procedures.