BALB/c和C_(57)BL/6小鼠在基因功能检测中行为学的比较研究

目的　探索BALB c和C57BL 6两个品系在有关实验中的不同作用。方法　选取了结合随机测序与生物信息学分析设计合成的神经系统表达的一些基因的反义核酸 (anti sense)中的 2个 ,用Hamilton微量注射器将其分别定量注射到BALB c和C57BL 6小鼠的侧脑室 ,并分别设注射生理盐水和随机序列核酸 (Scramble)的对照组。每一反义核酸实验组和对照组各注射 1 0只小鼠 ,之后观察实验组与对照组在不同行为学实验中的差异。小鼠的行为学检测模型为 :考察日常代谢能力的摄食量 ,考察Locomotionactivity(移动 )的旷场行为 ,考察疼痛阈值的甩尾试验和考察记忆能力的步下法实验。结果　注射No 1基因的反义核酸后 ,两品系的实验组均在测试记忆力的步下法 (Step -downTest)试验中表现出记忆力减弱 ,且与对照组差异明显 ,说明No 1基因的功能确与记忆相关。注射No 2基因的反义核酸后 ,在测试移动能力的旷场行为 (OpenFieldBehavior)试验中 ,BALB c实验组跨格、直立行为均比对照组明显减少 ,说明受此反义核酸影响显著 ,而C57BL 6实验组则与对照组无大的差异。此外 ,在生理盐水对照组和随机序列核酸对照组的实验中以及其他行为学模型的实验中 ,两品系也存在着一定的差异。结论　用遗传背景不同的多品系进行相关实验 ,可进一步建立

Comparison Study on Behavior of BALB/c and C_(57) BL/6 Mouse for Monitoring of Gene Function

Objective To study the different usage of BALB/c and C 57 BL/6,the two strains of laboratory mouse with different genetic backgrounds,in relevant experiments Methods Authors chose antisense oligo-nucleotides of two genes expressed in central nervous system by random sequencing and bioinformatics analysis, injected them into the lateral ventricles of mouse brain using Hamilton micro-syringe respectively There are also two control groups of saline and scramble Each group has ten mice And then several behavioral tests were carried out They are Feeding for metabolic ability,Open Field Behavior for locomotion activity,Tail Flick Latency(TFL)for limen of pain,Step-down Test for memory Results It was found that after the injection of No 1 antisense both two strains of mice showed damaged memory and there were obvious differences between experimental groups and control groups It is said that the function of No 1 gene is correlative with memory After the No 2 antisense was injected,BALB/c strain experimental group showed obvious decrease in numbers of step-across and standing-up in open field behavior test compared with control groups But in C 57 BL/6 mice,there weren't obvious differences between experimental group and control groups And there were also differences between the control groups of the two strains Conclusion The results show that using laboratory mouse strains with different genetic backgrounds can set up a further monitoring system for the screening of novel gene funtion Simultaneously the results have certain significance in finding which kinds of strains are suitable for which kinds of experiments