灵芝子实体和菌丝体的提取物及其各纯化组份生物活性的比较

从不同品种的灵芝中筛选出了多糖含量最高的菌株GL2为材料,利用柱层析技术从子实体和菌丝体提取物中分离得到多个组分。实验发现子实体组分P3,P31及P32对人白血病细胞株K562的生长有明显地抑制作用,这三个组分中只有P32对另一白血病细胞株HL-60有抑制作用。免疫活性测试的结果显示子实体各组分在刺激小鼠脾淋巴细胞,T和B细胞的增殖,提高人外周血中NK细胞杀伤活性方面比菌丝体的作用强;进一步的实验发现子实体与菌丝体相应组分在刺激人外周血中的T和B淋巴细胞增殖方面的活性差异不大。子实体和菌丝体提取物各组份均可剂量依赖型的促进PBMC分泌TNF-α。菌丝体提取物各组分对TNF-释放量的影响在低浓度时与子实体各组份相当,在高浓度时要明显好于赤芝子实体提取物各组分。

COMPARISON OF BIOACTIVITY OF FRUITING BODY AND MYCELIA OF GANODERMA LUCIDUM AND THEIR PURIFIED FRACTIONS

Ganoderma lucidum strain GL2 that had the highest content of polysaccharide was screened from several Ganoderma strains. Fractions were fractionated from extracts of fruit body and mycelia by different chromatography. P3,P31 and P32 significantly inhibited the proliferation of leukemia K-562 cells, and P32 also inhibited the proliferation of leukemia HL-60 cells. Effects of fractions of fruit body on stimulating the proliferation of spleen lymphocyte and T and B cells and activating the NK activity is stronger than that of fractions of mycelia. In addition, fractions of fruit body and mycelia have similar effect on stimulating the proliferation of T and B cells in peripheral blood mononuclear cells. Fractions of fruit body and mycelia induced peripheral blood mononuclear cells to release TNF- in dose-dependent manner.. At low concentration fractions of mycelia had similar capacity to induce the production of TNF-αwith fractions of fruit body; however, at high concentration, fractions of mycelia are better than that of fruit body.