Discovery and Analysis of MicroRNAs in Leymus chinensis under Saline-Alkali and Drought Stress Using High-Throughput Sequencing |
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| Authors: | Junfeng Zhai Yuanyuan Dong Yepeng Sun Qi Wang Nan Wang Fawei Wang Weican Liu Xiaowei Li Huan Chen Na Yao Lili Guan Kai Chen Xiyan Cui Meiying Yang Haiyan Li |
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| Institution: | 1. Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, China.; 2. College of Life Sciences, Jilin Agricultural University, Changchun, Jilin, China.; 3. High School attached to Northeast Normal University, Changchun, Jilin, China.; Universidade Federal do Rio Grande do Sul, Brazil, |
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| Abstract: | Leymus chinensis (Trin.) Tzvel. is a perennial rhizome grass of the Poaceae (also called Gramineae) family, which adapts well to drought, saline and alkaline conditions. However, little is known about the stress tolerance of L. chinensis at the molecular level. microRNAs (miRNAs) are known to play critical roles in nutrient homeostasis, developmental processes, pathogen responses, and abiotic stress in plants. In this study, we used Solexa sequencing technology to generate high-quality small RNA data from three L. chinensis groups: a control group, a saline-alkaline stress group (100 mM NaCl and 200 mM NaHCO3), and a drought stress group (20% polyethylene glycol 2000). From these data we identified 132 known miRNAs and 16 novel miRNAs candidates. For these miRNAs we also identified target genes that encode a broad range of proteins that may be correlated with abiotic stress regulation. This is the first study to demonstrate differentially expressed miRNAs in L. chinensis under saline-alkali and drought stress. These findings may help explain the saline-alkaline and drought stress responses in L. chinensis. |
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