Regulation of adiponectin on lipid metabolism in large yellow croaker (Larimichthys crocea) |
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| Affiliation: | 1. Institut des sciences de la mer de Rimouski, Université du Québec à Rimouski, 310 Allée des Ursulines, Rimouski, Québec G5L 3A1, Canada;2. Institut de Biologie Intégrative et des Systèmes, Département de biologie, Université Laval, 1030 avenue de la Médecine, Québec, Québec G1V 0A6, Canada;1. Post-Graduation Program in Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;2. BRAIN Laboratory (Basic Research and Advanced Investigations in Neurosciences) – Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil;3. Gynecological Endocrinology Unit, Division of Endocrinology, Hospital de Clinicas de Porto Alegre, Department of Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;4. Laboratory of Inborn Errors of Metabolism, Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, Brazil;5. Medical Genetics Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil;6. Laboratory for Clinical Biochemistry and Metabolism, University Medical Center, Freiburg, Germany;1. Department of Physiology and Immunology, Faculty of Biology, University of Barcelona, Av. Diagonal 643, Barcelona 08028, Spain;2. Nofima (Norwegian Institute of Food, Fisheries and Aquaculture Research), P.O. Box 210, NO-1431 Ås, Norway |
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| Abstract: | Adiponectin (APN), an adipose tissue-derived hormone, plays a key role in regulating energy metabolism in mammals. However, its physiological roles in teleosts remain poorly understood. In the present study, the apn gene was cloned from large yellow croaker, which was mainly expressed in the adipose, muscle and liver. Further studies showed that adaptor protein phosphotyrosine interaction PH domain and leucine zipper 1 (APPL1) was localized in the cytoplasm near the cell membrane and was directly bounded to adiponectin receptors (AdipoRs). Meanwhile, APN played a crucial role in lipid metabolism of primary muscle cells by promoting the synthesis, oxidation and transport of fatty acids, and the promoting effects were blocked by knockdown of appl1 and AdipoRs. Furthermore, the activation/inhibition of peroxisome proliferators activated receptor γ (PPARγ) enhanced/suppressed the APN-mediated lipid metabolism. Overall, results showed that APN mediated lipid metabolism through AdipoRs-APPL1 activated PPARγ and further regulated the synthesis, oxidation and transport of FA. This study will facilitate the investigation of APN functions in lipid metabolism and energy homeostasis and reveal the evolution of lipids utilization and energy homeostasis in vertebrates. |
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