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Nitrogen metabolism inErica and soybean,two species differing by their sensitivity to inorganic N source
Authors:G Guerrier
Institution:(1) FRD Biotechnologies, Ensemble Scientifique, 2 Bd Lavoisier, 490 45 Angers Cedex, France
Abstract:Growth of soybean was not altered, whatever the inorganic N-source (NO3, NH4 or a NO3/NH4 mixture); conversely, growth of Erica x darleyensis plants in vitro decreased more in. NH4 medium than in a NO3 medium, compared to a NO3/NH4 medium. The GS/GOGAT pathway (in NH4 medium), the nitrate and nitrite reductase activities (in NO3 medium), as the contents in free nitrogenous forms and total nitrogen (in NO3 and NH4 media) were not more altered in Erica than in soybean, compared to a NO3/NH4 medium. PEPCase activity was the highest in soybean irrespective of the N-treatments; the involvement of PEPCase in N-metabolism could be explained by its function in ionic and osmotic balances rather than its function in supplying carboxylates as acceptors for NH4-assimilation.
Keywords:Erica x darleyensis  Glycine max  ionic and osmotic balances  N-assimilation  N-reduction  stress
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