Influence of formamide on the thermal stability of DNA |
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| Authors: | Chanchal Sadhu Santanu Dutta K P Gopinathan |
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| Institution: | (1) Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560 012 Bangalore, India;(2) Molecular Biophysics Unit, Indian Institute of Science, 560 012 Bangalore, India |
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| Abstract: | The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex
DNA is lowered by 0·6°C per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide
also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation |
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| Keywords: | DNA melting formamide T m DNA base composition DNA reassociation |
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