Immunocytochemical localization of myosin in the brush border region of the intestinal epithelium |
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Authors: | Dr. Detlev Drenckhahn Renate Steffens Ute Gröschel-Stewart |
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Affiliation: | (1) Department of Anatomy, University of Kiel, Kiel, Germany;(2) Institute of Zoology, Technische Hochschule Darmstadt, Darmstadt, Germany;(3) Anatomisches Institut der Universität, Olshausenstr.40-60, D-2300 Kiel, Germany |
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Abstract: | Summary Myosin was localized in rat intestinal epithelium by means of indirect immunofluorescence and immunoelectron microscopy (unlabeled antibody peroxidase method), using a specific antibody to myosin from chicken gizzard. Immunoreactivity was localized in the apical cytoplasm, where it was concentrated along the rootlets of the microvillar filament bundles and in the terminal web. A model of microvillar contraction is proposed. |
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Keywords: | Myosin Intestinal epithelium Brush border Immunocytochemistry |
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