The enzymatic system thiosulfate: Cytochrome c oxidoreductase from photolithoautotrophically grown Chromatium vinosum |
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Authors: | Wolfgang Schmitt Guido Schleifer Karl Knobloch |
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Institution: | (1) Institut für Botanik und Pharmazeutische Biologie der Universität Erlangen-Nürnberg, Schlossgarten 4, D-8520 Erlangen, Germany |
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Abstract: | Chromatium vinosum cells form a vesicular type intracytoplasmic membrane system during phototrophic growth on thiosulfate.—An enzyme protein transferring electrons from thiosulfate to cytochromes of type c was enriched from S-144. The colorless thiosulfate: cytochrome c oxidoreductase was characterized by a molecular weight of 36,000 (after dodecylsulfate treatment) and 35,000 (by gel filtration). Isoelectric focusing revealed a pI range of 4.4 to 4.7. Apparent K
m values for the cytochromes tested were in the M range. — The endogenous electron acceptor compound, isolated from the chromatophore fraction P-144, was found to be a membrane-bound cytochrome c-552. The homogeneous cytochrome protein had an average pI value of 4.65 and a molecular weight of 71,500 determined by gel filtration. By dodecylsulfate electrophoresis it was cleaved into two proteins representing particle weights of 45,000 and 20,000.Abbreviations HiPIP
high potential nonheme iron protein
- IEF
isoelectric focusing
- SDS
dodecylsulfate, sodium salt
- Temed
N,N,N,N-tetramethylethylenediamine |
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Keywords: | Photosynthesis Chromatium vinosum Thiosulfate Cytochrome c Oxidoreductase |
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