Difference between polylysine and polyarginine in changing DNA structure upon complex formation |
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| Authors: | M Zama S Ichimura |
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| Affiliation: | 1. Department of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies, Yongin 449-791, Republic of Korea;2. Department of Chemical Engineering, Pukyong National University, Pusan 608-739, Republic of Korea;1. School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China;2. Analytical Instrumentation Center, Anhui University of Technology, Maanshan 243002, China;1. Laboratory of Biomedical Optics and Imaging, Federal University of Pernambuco, Recife, PE, Brazil;2. Biophysics and Radiobiology Department, Federal University of Pernambuco, Recife, PE, Brazil;3. Pharmaceutical Sciences Department, Federal University of Pernambuco, Recife, PE, Brazil;4. Centre for Telecommunication Studies, Pontifical Catholic University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil;1. State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;2. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
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| Abstract: | Soluble complexes of DNA with poly-L-lysine and poly-L-arginine were prepared by dialysis or mixing method, and their circular dichroism spectra and viscosities were measured. Both complexes formed by dialysis method had different circular dichroism spectra from that of free DNA respectively, i.e., the spectrum of the DNA-poly-L-lysine complex was similar to that of DNA at high salt concentration and the spectrum of the DNA-poly-L-arginine complex resembled that of DNA under melting at elevated temperature. The viscosity of the both complexes decreased pronouncedly with the increase in peptide to DNA ratio, although the viscosity of the DNA-poly-L-lysine complex was more rapidly decreased than that of the DNA-poly-L-arginine complex. The difference between the two polypeptides in changing DNA structure was observed also in the complexes formed by mixing method. |
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