A novel nucleolytic activity associated with rabbit liver tRNA nucleotidyltransferase |
| |
Authors: | M P Deutscher |
| |
Institution: | Department of Biochemistry University of Connecticut Health Center Farmington, Connecticut 06032 USA |
| |
Abstract: | Purified preparations of rabbit liver tRNA nucleotidyltransferase contain a nucleolytic activity which removes terminal CMP residues from tRNA-C-C and tRNA-C-C-C. Other tRNA molecules, such as tRNA-C-C-A, tRNA-C-A, tRNA-C-U and tRNA-C are not substrates for this reaction. The activity exhibits a sharp optimum at about pH 10 and a divalent cation (Mg++ or Mn++) is required. The reaction is inhibited by ATP, CTP, pyrophosphate and potassium chloride. The relation of this activity to other reactions catalyzed by tRNA nucleotidyltransferase is discussed. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|