Incorporation of β-amyloid protein through the bovine ileal epithelium before and after weaning: Model for orally transmitted amyloidoses |
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| Authors: | Yasuhisa Ano Hiroyuki Nakayama Yusuke Sakai Akikazu Sakudo Maiko Endo Shogo Ebisu JunYou Li Koji Uetsuka Noboru Manabe Takashi Onodera |
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| Institution: | Department of Molecular Immunology;, Department of Veterinary Pathology;, and Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo;, Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Ibaraki-Kasama;, Research Institute of Microbial Diseases, Osaka University, Osaka;and Research and Development, Business Development Protein Express Co., Chiba, Japan |
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| Abstract: | To determine the mechanism of bovine intestinal incorporation of the pathogen, and the pathogenesis of prion protein in the early stage, cows suckling and weaning were orally given a fusion protein of Aβ-EGFP. Aβ-EGFP was incorporated through the villous columnar epithelial cells and accumulated in crypt patches in the ileum of suckling cows. The sites of the uptake and accumulation of Aβ-EGFP are very close to the peripheral nervous system; however, such uptake of Aβ-EGFP was not observed in 6-month-old post-weaning cows. The present study, therefore, suggests that the weaning period is very important for the risk of transmission. |
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| Keywords: | β-amyloid protein bovine spongiform encephalopathy suckling period uptake |
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