Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization |
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| Authors: | Bong-Gyu Kim Na Yeon Kim Jeong Ho Kim Kazuya Akimitsu Youhoon Chong Joong-Hoon Ahn |
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| Affiliation: | (1) Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul, 143-701, South Korea;(2) Laboratory of Plant Pathology, United Graduate School and Faculty of Agriculture, Kagawa University, Miki Kagawa, 761-0795, Japan |
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| Abstract: | Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteolin, quercetin, and tricin reaction products using liquid chromatography-mass spectrometry revealed that these were diglucosylated. The glucosylation positions of kaempferol, which was the best substrate, were determined to be the 3- and 7-hydroxyl groups. This is the first flavonoid O-diglucosyltransferase described from rice. |
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| Keywords: | Flavonoids Flavonoid O-diglucoside Glycosyltransferase Oryza sativa UGT |
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