Specific interaction of TFII-I with an upstream element on the HIV-1 LTR regulates induction of latent provirus |
| |
| Authors: | Malcolm Tom Kam Joanna Pour Pouya Sadeghi Sadowski Ivan |
| |
| Affiliation: | Department of Biochemistry and Molecular Biology, Molecular Epigenetics, LSI, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3 |
| |
| Abstract: | RBF-2 is a factor comprised of a USF1/2 heterodimer, whose association with a highly conserved upstream element (RBEIII) on the HIV-1 LTR requires a co-factor TFII-I. We have identified specific nucleotides, immediately 3′ of RBEIII that are required for stable association of TFII-I with this region of the LTR. Mutations that inhibit interaction of TFII-I with DNA also prevent stimulation of USF binding to RBEIII, and render the integrated LTR unresponsive to T cell signaling. These results demonstrate an essential role of TFII-I bound at an upstream LTR element for viral replication. |
| |
| Keywords: | HIV-1 TFII-I USF RBF-2 MAPK signaling |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
