Recombinant subunits as tools for the structural and functional characterization of Echinococcus granulosus antigen B |
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| Authors: | Monteiro Karina M Zaha Arnaldo Ferreira Henrique B |
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| Institution: | a Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Cx. Postal 15005, 91501-970 Porto Alegre, RS, Brazil
b Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil |
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| Abstract: | Antigen B (AgB) is a major protein component of the Echinococcus granulosus metacestode. It is oligomeric and this raises several questions regarding the subunit structure and composition of AgB. Several genes that encode different AgB subunits have been identified, and some of these have been cloned and expressed to produce recombinant subunits. The study of these recombinant subunits may provide new insights into the structure, physical-chemical properties, and functional aspects of AgB. Like native AgB, the AgB8/1, AgB8/2, and AgB8/3 recombinant subunits produced in our laboratory form 120-160 kDa oligomers that have stable secondary structures, are strongly antigenic and immunogenic, and selectively bind hydrophobic compounds. Here, we review these results and discuss their implications for the elucidation of the structure and function of AgB. This includes a possible role for AgB in host-parasite interactions. |
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| Keywords: | 2DE two-dimensional gel electrophoresis AFM atomic force microscopy AgB antigen B CD circular dichroism CDS coding DNA sequence CHD cystic hydatid disease DLS dynamic light scattering ESI-Q-TOF MS electrospray ionization quadrupole time-of-flight mass spectrometry GST glutathione S-transferase HLBP helix-rich hydrophobic ligand binding protein MALDI-TOF MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry MALDI-TOF MS/MS matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry MS mass spectrometry SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis TEM transmission electron microscopy UTR untranslated region Cestode Recombinant antigen Cystic hydatid disease Host-parasite interaction Protein structure |
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