Neospora caninum: detection in wild rabbits and investigation of co-infection with Toxoplasma gondii by PCR analysis |
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Authors: | Hughes J M Thomasson D Craig P S Georgin S Pickles A Hide G |
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Affiliation: | a Centre for Parasitology and Infectious diseases, Biomedical Sciences Research Institute, University of Salford, Salford M5 4WT, UK b Field Studies Council at Malham Tarn, Settle, North Yorkshire BD24 9PU, UK |
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Abstract: | Neospora caninum is an important pathogen of cattle causing significant economic loss. There is much current interest in wild animal reservoirs for this parasite. The role of the rabbit in this is currently unknown. DNA samples from the brains of wild rabbits (Oryctolagus cuniculus) collected from the Malham area of the Yorkshire dales were investigated by species-specific PCR for the presence of N. caninum and Toxoplasma gondii. We found prevalences of N. caninum of 10.5% (6/57) and T. gondii of 68.4% (39/57) with 8.8% (5/57) co-infected. Strain typing of T. gondii positive rabbits revealed strain types I-III were present in this population. Investigation of tissue distribution determined N. caninum DNA was most often detected in the brain and heart, less often in the tongue and not in the liver. To our knowledge this is the first report of N. caninum detection in naturally infected wild rabbits. |
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Keywords: | Apicomlexa Neospora caninum Toxoplasma gondii PCR Co-infection Rabbit Wildlife DNA, deoxyribonucleic acid ELISA, enzyme linked immunosorbent assay IFAT, indirect florescent test PCR, polymerase chain reaction SAG, surface antigen gene TE, Tris-EDTA buffer |
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