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Nepmucin/CLM-9, an Ig domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration in vitro |
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| Authors: | Jin Soojung Umemoto Eiji Tanaka Toshiyuki Shimomura Yoshimitsu Tohya Kazuo Kunizawa Keiji Yang Bo-Gie Jang Myoung Ho Hirata Takako Miyasaka Masayuki |
| Institution: | a Laboratory of Immunodynamics, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan b Laboratory of Immunodynamics, WPI Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan c Laboratory of Immunobiology, Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, Kobe 650-8530, Japan d Department of Anatomy, Kansai University of Health Sciences, Osaka 590-0482, Japan e Laboratory of Gastrointestinal Immunology, WPI Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan f Laboratory of Combined Research on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan |
| Abstract: | Nepmucin/CLM-9 is an Ig domain-containing sialomucin expressed in vascular endothelial cells. Here we show that, like CD31, nepmucin was localized to interendothelial contacts and to vesicle-like structures along the cell border and underwent intracellular recycling. Functional analyses showed that nepmucin mediated homotypic and heterotypic cell adhesion via its Ig domain. Nepmucin-expressing endothelial cells showed enhanced lymphocyte transendothelial migration (TEM), which was abrogated by anti-nepmucin mAbs that block either homophilic or heterophilic binding. Notably, the mAbs that inhibited homophilic binding blocked TEM without affecting lymphocyte adhesion. These results suggest that endothelial nepmucin promotes lymphocyte TEM using multiple adhesion pathways. |
| Keywords: | LNs lymph nodes HEVs high endothelial venules PPs Peyer&rsquo s patches LBRC lateral border recycling compartment MBEC4 mouse brain endothelial cell 4 EC endothelial cell TEM transendothelial migration |
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