AFLP analysis to assess genomic stability in Solanum regenerants derived from wild and cultivated species |
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Authors: | Riccardo Aversano Francesco Di Dato Antonio Di Matteo Luigi Frusciante Domenico Carputo |
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Institution: | (1) Department of Soil, Plant, Environmental and Animal Production Sciences, University of Naples “Federico II”, Via Universit? 100, 80055 Portici, Italy; |
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Abstract: | The cultivated potato as well as its tuber-bearing relatives are considered model plants for cell and tissue culture, and
therefore for exploiting the genetic variation induced by in vitro culture. The association between molecular stability and
tissue culture in different genetic backgrounds and ploidy levels has already been explored. However, it still remains to
be ascertained whether somaclonal variation differs between callus-derived chromosome-doubled and undoubled regenerants. Our
research aimed at investigating, through amplified fragment length polymorphism (AFLP) markers, the genetic changes in marker-banding
patterns of diploid and tetraploid regenerants obtained from one clone each of Solanum bulbocastanum Dunal and S. cardiophyllum Lindl (both 2n = 2x = 24) and tetraploids from cultivated S. tuberosum L. (2n = 4x = 48). Pairwise comparisons between the banding patterns of regenerants and parents allowed detecting considerable changes
associated to in vitro culture both at diploid and tetraploid level. The percentages of polymorphic bands between diploid
and tetraploid regenerants were, respectively, 57 and 69% in S. bulbocastanum and 58 and 63% in S. cardiophyllum. On average, the frequencies of lost parental fragments in regenerants were significantly higher than novel bands both in
S. bulbocastanum (48 vs. 22%) and S. tuberosum (36 vs. 18%) regenerants. By contrast, in S. cardiophyllum, a similar incidence of the two events was detected (32 vs. 29%). Our results revealed that structural changes after tissue
culture process strongly affected the genome of the species studied, but diploid and tetraploids regenerated plants responded
equally. |
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