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Sarcocystis neurona: molecular characterization of enolase domain I region and a comparison to other protozoa |
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| Authors: | Bolten K E Marsh A E Reed S M Dubey J P Toribio R E Saville W J A |
| Institution: | a Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 601 Vernon Tharp Street, Columbus, OH 43210, USA b Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1920 Coffey Road, Columbus, OH 43210, USA c Rood & Riddle Equine Hospital, 2150 Georgetown Road, Lexington, KY 40511, USA d US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC-East, Beltsville, MD 20705, USA |
| Abstract: | Sarcocystis neurona causes protozoal myeloencephalitis and has the ability to infect a wide host range in contrast to other Sarcocystis species. In the current study, five S. neurona isolates from a variety of sources, three Sarcocystis falcatula, one Sarcocystis dasypi/S. neurona-like isolate, and one Besnoitia darlingi isolate were used to compare the enolase 2 gene segment containing the domain I region to previously sequenced enolase genes from Neospora caninum, Neospora hughesi, Toxoplasma gondii, Plasmodium falciparum, and Trypanosoma cruzi; enolase 2 segment containing domain I region is highly conserved amongst these parasites of veterinary and medical importance. Immunohistochemistry results indicates reactivity of T. gondii enolase 1 and 2 antibodies to S. neurona merozoites and metrocytes, but no reactivity of anti-enolase 1 to the S. neurona bradyzoite stage despite reactivity to T. gondii bradyzoites, suggesting expression differences between organisms. |
| Keywords: | Protozoal myeloencephalitis Sarcocystis neurona Sarcocystis falcatula Sarcocystis dasypi Besnoitia darlingi Enolase |
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