药物诱导的玉米根尖凋亡细胞表面电特性研究(英文)

细胞凋亡过程中细胞表面膜的电位很可能会发生改变。本文首次报导：应用细胞电泳技术（ｃｅｌｌ ｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ）对细胞毒素类药物放线菌酮（ｃｙｃｌｏｈｅｘｉｍｉｄｅ）、放线菌素 Ｄ（ａｃｔｉｎｏｍｙｃｉｎ Ｄ）和秋水仙碱（ｃｏｌｃｈｉｃｉｎｅ）等诱导的植物凋亡细胞与正常细胞之间电泳迁移率（ＥＰＭ）的差异进行了比较，对引起的膜电位变化进行了定量分析。实验以玉米根尖分生组织为材料，制备原生质体，经过适当剂量的药物处理（Ｆｉｇ．１－Ｂ），在尽量减少细胞膜被破坏的情况下（Ｆｉｇ．２），观察到：三种细胞毒素类药物的作用有所不同，被诱导的植物凋亡细胞的膜表面Ｚｅｔａ电位绝对值比正常细胞的高（Ｆｉｇ．１－Ａ）。本研究提示细胞电泳可对凋亡细胞表面膜电位的变化进行定量分析，为细胞凋亡的检测在方法上提供了新思路。

Electrical Characters on Surface of Apoptotic Cell in Maize Roots Induced by Cytotoxins

The difference of electrophoretic mobility (EPM) value between normal and apoptotic cells induced by cytotoxin, cycloheximide, actinomycin D or colchicine, was identined for the first time by using the method of cell electrophoresis. Combined with protoplast preparation, the change of electric charge on protoplast membrane of apoptotic cells was quantified directly in an in vivo system of the root tip of maize. Experimental results indicated that the absolute value of Zeta potential on the membrane of apoptotic plant cells induced by cytotoxic agents was much higher than that in normal cells. The presented results indicated that the method of cell electrophoresis could be thought as a creative approach to accomplish quantitative analysis of surface electric charge of cell membrane of apoptotic cells.