| Glycogen synthase kinase-3β positively regulates the proliferation of human ovarian cancer cells |
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| 引用本文： | Cao Q,Lu X,Feng YJ. Glycogen synthase kinase-3β positively regulates the proliferation of human ovarian cancer cells[J]. Cell research, 2006, 16(7): 671-677 |
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| 作者姓名： | Cao Q Lu X Feng YJ |
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| 作者单位： | Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China |
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| 基金项目： | Acknowledgments We thank Dr Y Rao （Northwestem University） for generously providing Axin GID5-6/pCS2MT, Axin GID5- 6LP/pCS2MT and GSK-313 S9A/pCS2 plasmids and YC Jia, CX Huang, Y Hou for critical discussion and ZJ Fan for technical support. This work was supported by Grant from Shanghai Foundation for Key Subject Development of Medical Science （05111016）. |
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| 摘 要： | Although glycogen synthase kinase-3 （GSK-3） might act as a tumor suppressor since its inhibition is expected to mimic the activation of Wnt-signaling pathway, GSK-3β may contribute to NF-κB activation in cancer cells leading to increased cancer cell proliferation and survival. Here we report that GSK-3β activity was involved in the proliferation of human ovarian cancer cell both in vitro and in vivo. Inhibition of GSK-3 activity by pharmacological inhibitors suppressed proliferation of the ovarian cancer cells. Overexpressing constitutively active form of GSK-3β induced entry into the S phase, increased cyclin D1 expression and facilitated the proliferation of ovarian cancer cells. Furthermore, GSK-3 inhibition prevented the formation of the tumor in nude mice generated by the inoculation of human ovarian cancer cells. Our findings thus suggest that GSK-3β activity is important for the proliferation of ovarian cancer cells, implicating this kinase as a potential therapeutic target in ovarian cancer.
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| 关 键 词： | 糖原合酶激酶-3β 酶活性 卵巢肿瘤 肿瘤细胞 |
| 收稿时间： | 2005-12-15 |
| 修稿时间： | 2005-12-152006-04-24 |
Glycogen synthase kinase-3beta positively regulates the proliferation of human ovarian cancer cells |
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| Cao Qi,Lu Xin,Feng You-Ji. Glycogen synthase kinase-3beta positively regulates the proliferation of human ovarian cancer cells[J]. Cell research, 2006, 16(7): 671-677 |
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| Authors: | Cao Qi Lu Xin Feng You-Ji |
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| Affiliation: | Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China. |
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| Abstract: | Although glycogen synthase kinase-3 (GSK-3) might act as a tumor suppressor since its inhibition is expected to mimic the activation of Wnt-signaling pathway, GSK-3beta may contribute to NF-kappaB activation in cancer cells leading to increased cancer cell proliferation and survival. Here we report that GSK-3beta activity was involved in the proliferation of human ovarian cancer cell both in vitro and in vivo. Inhibition of GSK-3 activity by pharmacological inhibitors suppressed proliferation of the ovarian cancer cells. Overexpressing constitutively active form of GSK-3beta induced entry into the S phase, increased cyclin D1 expression and facilitated the proliferation of ovarian cancer cells. Furthermore, GSK-3 inhibition prevented the formation of the tumor in nude mice generated by the inoculation of human ovarian cancer cells. Our findings thus suggest that GSK-3beta activity is important for the proliferation of ovarian cancer cells, implicating this kinase as a potential therapeutic target in ovarian cancer. |
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