| 应用原位杂交技术检测X射线诱发的特定染色体的微核率 |
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| 引用本文： | 余从年,方思鸣,周光云,A.Wojcik,C．Streffer. 应用原位杂交技术检测X射线诱发的特定染色体的微核率[J]. 中国组织化学与细胞化学杂志, 1998, 0(2) |
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| 作者姓名： | 余从年 方思鸣 周光云 A.Wojcik C．Streffer |
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| 作者单位： | 同济医科大学细胞生物学教研室!武汉，430030，同济医科大学细胞生物学教研室!武汉，430030，同济医科大学细胞生物学教研室!武汉，430030，德国埃森大学医学院医学放射生物学研究所，德国埃森大学医学院医学放射生物学研究所 |
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| 摘 要： | 为了研究X射线与X染色体的微校率之间的关系．本实验利用原位杂交技术同时检测了经X射线诱发人双核淋巴细胞的7号和X染色体的微核率。结果发现：经2．5Gy的X射线照射后．X和7号染色体的微核率男性分别为3．4％和7．1％；女性分别为6．6％和6．0％。X和7号染色体微核率的实验观察值与理论预期值之间在统计学上无显著性差异。实验结果提示：X射线并不特异性引起X染色体的微核率增高。
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| 关 键 词： | 7号染色体 X染色体 X射线 微核 人淋巴细胞 原位杂交技术。 |
DETECTION OF THE MICRONUCLEUS FREQUENCY OF SPECIFIC CHROMOSOME INDUCED BY X-RAYS USING WHOLE CHROMOSOME PAINTING UETECHNIQUE |
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| Yu Congnian,Fang Siming,Zhou Guangyun,A Wsojcik,CStreffer. DETECTION OF THE MICRONUCLEUS FREQUENCY OF SPECIFIC CHROMOSOME INDUCED BY X-RAYS USING WHOLE CHROMOSOME PAINTING UETECHNIQUE[J]. Chinese Journal of Histochemistry and Cytochemistry, 1998, 0(2) |
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| Authors: | Yu Congnian Fang Siming Zhou Guangyun A Wsojcik CStreffer |
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| Abstract: | To study the relations between X-ray and the frequency of micronuclei with X-chromosome, the occurrence of Chromosome 7 and X chromosome within micronuclei induced by Xrays wa.s simultaneously scored in binucleated human lymphocytes using whole chromosome painting technique. After X-ray exposure (2. 5 Gy) the frequency of micronuclei contained X-chromosome and chromosome 7 is 3. 4% and 7.1% in male 6' 6% and 6. 0% in female.There are no statistically significant differences in the frequency between theoretically expected and experimentally found inclusion of X-chromosome or chromosome 7 in micronuclei. These results suggest that X-ray does not increase X chromosome in micronuclei preferentially. |
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| Keywords: | Chromosome 7 X-chromosome X-ray Microntlclei: Human lymphocyte |
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