Plant cells show an acquired insensitivity to Brefeldin A |
| |
Authors: | Steele-King C |
| |
Institution: | Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Headington, Oxford OX3 0BP, UK; Institut des Sciences Végétales, CNRS, UPR 40, Cytométrie, 91198 Gif sur Yvette, France; Corresponding author at: Centre for Plant Sciences, Leeds Institute for Plant Biotechnology and Agriculture, University of Leeds, Leeds LS2 9JT, UK. E-mail: bmbcgsk@bmb.leeds.ac.uk |
| |
Abstract: | The parameters of Brefeldin A (BFA) action in plant cells have been further
investigated in maize root tips. Incubation of maize root tip cells with
100 g ml-1 BFA for 2 h
induces the vesiculation and aggregation of Golgi membrane to form BFA
compartments; an effect that is reversed by incubation of the cells in
BFA-free medium. In this paper, the effects of prolonged incubation with
BFA are reported, revealing that Golgi stacks spontaneously re-form in the
presence of this drug after incubation for 12 h or 24 h. The observation
that the same BFA solution is still able to induce the formation of BFA
compartments in fresh roots suggests that this recovery is not due to
metabolism, detoxification or depletion of the drug. Furthermore,
incubation of maize roots with a fresh 100 g
ml-1 BFA solution for 2 h following 24 h BFA
treatment fails to induce the reformation of BFA compartments. Thus, an
acquired insensitivity to BFA in plant cells, that has not been observed in
mammalian cells to date, is indicated. |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|