Aurora-A激酶对急性胰腺炎大鼠肺脏损伤的修复作用研究

摘要 目的：探讨与研究Aurora-A激酶对急性胰腺炎大鼠肺脏损伤的修复作用。方法：36只雄性SD大鼠均分为三组：对照组、模型组与Aurora-A组。对照组进行假手术操作，模型组建立急性胰腺炎模型后给予注射等量生理盐水治疗，Aurora-A组建立急性胰腺炎模型后给予阴茎背静脉注射鼠Aurora-A类因子-MLN8054 10 mg/kg治疗，记录大鼠肺脏损伤的修复情况。结果：造模过程中无大鼠死亡情况发生，模型组与Aurora-A组造模后2 w与4 w的肺组织病理评分、血清中性粒细胞弹性蛋白酶(neutrophil elastase，NE)与髓过氧化物酶(myeloperoxidase，MPO)含量、肺组织W/D、肺组织蛋白激酶B(AKT)、细胞外信号调节激酶1(ERK1)蛋白相对表达水平都高于对照组(P<0.05)，Aurora-A组少于模型组(P<0.05)。结论：Aurora-A激酶在急性胰腺炎大鼠的应用能抑制Akt/ERK信号通路激活，减少血清NE与MPO的表达，从而促进肺脏损伤修复。

Study on the Repairing Effect of Aurora-A Kinase on Lung Injury in Rats with Acute Pancreatitis

ABSTRACT Objective: To explore and study the repair effect of Aurora-A kinase on lung injury in rats with acute pancreatitis. Methods: 36 cases of male SD rats were equally divided into three groups-control group, model group and Aurora-A group. The control group were given underwent sham operation, the model group were treated with the same amount of normal saline after the acute pancreatitis model were established, and the Aurora-A group were treated with the mouse Aurora-A factor-MLN8054 10 mg/kg after the acute pancreatitis model were established. Recorded the repair effects of rat lung injury. Results: There were no rats died during the modeling process. The lung tissue pathology scores, serum neutrophil elastase (NE) and myeloperoxidase(MPO), lung tissue W/D, lung tissue protein kinase B (AKT), and extracellular signal-regulated kinase 1 (ERK1) protein in the model group and Aurora-A group at 2 weeks and 4 weeks after modeling were higher than those in the control group(P<0.05), the Aurora -A group were less than the model group (P<0.05). Conclusion: The application of Aurora-A kinase in acute pancreatitis rats can inhibit the activation of Akt/ERK signaling pathway, reduce the expression of serum NE and MPO, and promote lung injury repair.